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primescripttm fast rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm fast rt reagent kit
    Primescripttm Fast Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+fast+rt+reagent+kit/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pmc12757460-87-6-14
    Average 99 stars, based on 432 article reviews
    primescripttm fast rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: ASGR1 induces neutrophil extracellular traps formation to exacerbate sepsis-induced myocardial injury and cardiac dysfunction through SLC7A11 ubiquitination
    Article Snippet: 318 RNA isolation and quantitative real-time PCR 319 Total ribonucleic acid (RNA) was extracted using TRIzol reagent (Cat#15596018; Invitrogen Life 320 Technologies, Carlsbad, CA, USA) from tissues according to the manufacturer's instructions. .. Next, cDNA was 321 synthesized from 1 μg of total RNA using a PrimeScriptTM FAST RT reagent Kit with gDNA Eraserit (Takara, 322 Japan). .. Quantitative real-time PCR (qRT-PCR) was conducted by PrimeScriptTM II High Fidelity RT-PCR Kit 323 Takara, Japan) as previously described.

    Article Title: Dynamic changes in genes and metabolites during embryonic development of Jiangshan black-bone chickens
    Article Snippet: .. cDNA was synthesized from total RNA using the PrimeScriptTM FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, China). .. Gene-specific primers, listed in , were designed via the NCBI Primer-BLAST tool, with GAPDH serving as the internal housekeeping gene for normalization. qRT-PCR was conducted on a QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, USA) utilizing Universal SYBR® Green qPCR Master Mix (HY-K0501A, MCE, USA).

    Reverse Transcription:

    Article Title: Post-transcriptional effects of leptin 3′UTR polymorphisms on Milk traits in dairy cattle
    Article Snippet: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription was performed using the PrimeScriptTM FAST RT Reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer's instructions. .. Quantitative real-time PCR (qRT-PCR) was carried out using the TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Dalian, China) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition
    Article Snippet: Samples were observed using an upright microscope (NIKON ECLIPSE C1), and images were processed (coloring and merging) with Slide Viewer software. .. Total RNA was isolated from HD-11 cells and tissues using Trizol reagent (TaKaRa, Dalian, China) and reverse transcribed with the PrimeScriptTM Fast RT Reagent Kit with gDNA Eraser (TaKaRa). ..

    Article Title: SMPD1 modulates malignant progress of osteosarcoma through ferroptosis pathway.
    Article Snippet: J ur na l P re -p ro of

    Article Title: Critical roles of CD4-1 + T cells and their secreted IL-21 in vaccine-induced humoral immunity in grass carp.
    Article Snippet: Although CD4 T cells are known to play a crucial helper role in mammalian humoral immunity, the function of early-evolved CD4-1 T cells in teleost fish for assisting B cell-mediated antibody production remain to be elucidated.. Using grass carp (Ctenopharyngodon idella) as a model, this study demonstrated that CD4-1 T cells play a critical role in vaccine-induced antibody production and antibacterial immunity.. We confirmed that inactivated Aeromonas hydrophila vaccine significantly induced interleukin-21 (IL-21) expression in grass carp head kidney leukocytes (HKLs), and identified CD4-1 T cells as the primary cellular source of IL-21.

    Isolation:

    Article Title: Mechanism of Scutellaria baicalensis extracellular vesicles in attenuating Mycoplasma gallisepticum -induced inflammation via TRPC1 - STIM1/ORAI1 channel inhibition
    Article Snippet: Samples were observed using an upright microscope (NIKON ECLIPSE C1), and images were processed (coloring and merging) with Slide Viewer software. .. Total RNA was isolated from HD-11 cells and tissues using Trizol reagent (TaKaRa, Dalian, China) and reverse transcribed with the PrimeScriptTM Fast RT Reagent Kit with gDNA Eraser (TaKaRa). ..



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    TaKaRa primescripttm fast rt reagent kit
    Primescripttm Fast Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescripttm rt reagent kit
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    primescripttm rt reagent kit - by Bioz Stars, 2026-09
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